Representative flow cytometry plots from DAPI experiments. (A) Laminin cells with no DAPI are shown. Using the DAPI fluorescence of the cells, a gate was identified (line) to separate DAPI positive and DAPI negative cells. Applying this gate to the cells stained with DAPI (B) 1.02% of the cells were found to be DAPI positive/dead. (C) Neurosphere assay (NSA) cells with no DAPI are shown. Using the DAPI fluorescence of the cells, a gate was identified (line) to separate DAPI positive and DAPI negative cells. Applying this gate to the cells stained with DAPI (D) 1.14% of the cells were found to be DAPI positive/dead.