Predicting not only what will happen, but also when it will happen is extremely helpful for optimizing perception and action. Temporal predictions driven by periodic stimulation increase perceptual sensitivity and reduce response latencies. At the neurophysiological level, a single mechanism has been proposed to mediate this twofold behavioral improvement: the rhythmic entrainment of slow cortical oscillations to the stimulation rate. However, temporal regularities can occur in aperiodic contexts, suggesting that temporal predictions per se may be dissociable from entrainment to periodic sensory streams. We investigated this possibility in two behavioral experiments, asking human participants to detect near-threshold auditory tones embedded in streams whose temporal and spectral properties were manipulated. While our findings confirm that periodic stimulation reduces response latencies, in agreement with the hypothesis of a stimulus-driven entrainment of neural excitability, they further reveal that this motor facilitation can be dissociated from the enhancement of auditory sensitivity. Perceptual sensitivity improvement is unaffected by the nature of temporal regularities (periodic vs aperiodic), but contingent on the co-occurrence of a fulfilled spectral prediction. Altogether, the dissociation between predictability and periodicity demonstrates that distinct mechanisms flexibly and synergistically operate to facilitate perception and action.SIGNIFICANCE STATEMENTTemporal predictions are increasingly recognized as fundamental instruments for optimizing performance, enabling mammals to exploit regularities in the world. However, the notion of temporal predictions is often confounded with the idea of entrainment to periodic sensory inputs. At the behavioral level, it is also unclear whether perceptual sensitivity and reaction time improvements benefit the same way from temporal predictions and periodic stimulation. In two behavioral experiments on human participants, we find that periodic stimulation facilitates response readiness, whereas temporal predictions improve the precision of auditory processing. This latter effect arises regardless of the nature of temporal regularities (periodic vs aperiodic), but depends on the co-occurrence of a fulfilled spectral prediction.