GN, B1, B2, B5, and B7 tags in the unprimed conformation and the position of GN in the primed conformation are illustrated here for a rotation (θ) between top and right views of 90°. The direction of movement of GN between the primed and unprimed conformations relative to top and right views is shown. The magnitude of the 3D movement of GN is between 18.8 and 21.1nm (for θ lying between 50° and 116°).