Optimizing sample preparation for the isotopic measurement of 10Be extracted from quartz mineral separates has a direct positive effect on the efficiency of sample production and the accuracy and precision of isotopic analysis. Here, we demonstrate the value of tracing Be throughout the extraction process (both after dissolution and after processing), producing pure Be (by optimizing column chromatography methods and quantifying quartz mineral separate purity), and minimizing backgrounds (through reducing both laboratory process blanks and 10B isobaric interference). These optimization strategies increase the amount of 10Be available for analysis during accelerator mass spectrometry (AMS), while simultaneously decreasing interference and contamination, and ensuring that sample performance matches standard performance during analysis. After optimization of our laboratory’s extraction methodology, 9Be3+ beam currents, a metric for sample purity and Be yield through the extraction process, matched the 9Be3+ beam currents of AMS standards analyzed at the same time considering nearly 800 samples. Optimization of laboratory procedures leads to purer samples that perform better, more consistently, and more similarly to standards during AMS analysis, allowing for improved dating and quantification of Earth surface processes.