Figure 1. Maternal age relationships in more than 18,000 day-5 preimplantation embryos tested with based preimplantation genetic screening (PGS) based on 24-chromosome single-nucleotide polymorphism (SNP). (A) The number of embryos per in vitro fertilization (IVF) cycle submitted for testing gradually decreases as maternal age increases from 25 to 45 years old. Data points indicate the 50th percentile, and whiskers above and below these points correspond to the 75th and 25th percentiles, respectively. Age groups with less than 45 cycles are not shown. (B, C) On the basis of PGS results, embryos from both egg donors and nondonors were classified as either euploid or aneuploid. Women 24 to 35 years old have the highest percentage of euploid embryos. After age 35, mean euploidy rates decline with increasing maternal age, as shown by the close fit between these data points and the dashed regression line (C). The strong linear correlation (R2 = 0.981) indicates that the equation for this line can be used to accurately calculate (interpolate) average euploidy rates in women 35 to 45 years old. (B) Data points and whiskers show the mean and 1 standard deviation (SD), respectively. The logarithm of the number of embryos in each age group is mapped onto a color gradient from red (small n) to purple (large n). Age groups with less than 10 cycles are not shown.