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EMBO Press, The EMBO Journal, 22(25), p. 5358-5371, 2006

DOI: 10.1038/sj.emboj.7601392

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Replication of vertebrate mitochondrial DNA entails transient ribonucleotide incorporation throughout the lagging strand

Journal article published in 2006 by Takehiro Yasukawa, Aurelio Reyes, Tricia J. Cluett, Ming-Yao Yang ORCID, Mark Bowmaker, Howard T. Jacobs, Ian J. Holt
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Using two-dimensional agarose gel electrophoresis, we show that mitochondrial DNA (mtDNA) replication of birds and mammals frequently entails ribonucleotide incorporation throughout the lagging strand (RITOLS). Based on a combination of two-dimensional agarose gel electrophoretic analysis and mapping of 5′ ends of DNA, initiation of RITOLS replication occurs in the major non-coding region of vertebrate mtDNA and is effectively unidirectional. In some cases, conversion of nascent RNA strands to DNA starts at defined loci, the most prominent of which maps, in mammalian mtDNA, in the vicinity of the site known as the light-strand origin.