Although Unionidae mussels produce large biomass and reach high density in freshwater habitats, little is known about their ecology. We studied the distribution of 5 species of freshwater unionids in a eutrophic floodplain lake, on transects, along the lake shore and across the depth gradient. The clam distribution within the water body was not random: all species form a crowded zone along the lake shore, showing the highest density at ca. 0.5 m depth. The distribution of the most numerous species changed along the shore in Anodonta anatina and Unio pictorum but not in A. cygnea, whose numbers remained constant. The population numbers of the most numerous species showed a positive correlation with silt layer thickness. The generalized model of all the analyzed factors influencing the unionids’ distribution confirmed this relation and indicated a trade-off between water depth and distance from bank, which might be responsible for the occurrence of the zone at some optimum depth. Unionids have an important influence on freshwater ecosystem functions, thus their zonation implies that their functions are also spatially structured.