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Elsevier Masson, Analytical Biochemistry, 2(174), p. 662-665
DOI: 10.1016/0003-2697(88)90070-x
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Dot-blot assays and their use as a direct antigen-binding method to screen monoclonal antibodies to 1,4-β-and 1,3-β-glucan synthases
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Abstract
A rapid method has been developed to assay beta-glucan synthases spotted on a nitrocellulose sheet. The sensitivity of this method allows screening of hybridoma-making monoclonal antibodies in a direct antigen-binding assay by measurement of the activity of the enzymes retained by the antibodies previously fixed on nitrocellulose.