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eLife Sciences Publications, eLife, (5), 2016

DOI: 10.7554/elife.10051

Biophysical Society, Biophysical Journal, 3(110), p. 515a

DOI: 10.1016/j.bpj.2015.11.2753

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Stepwise Nucleosome Translocation by RSC Remodeling Complexes

Journal article published in 2016 by Bryan T. Harada ORCID, William L. Hwang, Sebastian Deindl, Nilanjana Chatterjee, Blaine Bartholomew, Xiaowei Zhuang
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

The SWI/SNF-family remodelers regulate chromatin structure by coupling the free energy from ATP hydrolysis to the repositioning and restructuring of nucleosomes, but how the ATPase activity of these enzymes drives the motion of DNA across the nucleosome remains unclear. Here, we used single-molecule FRET to monitor the remodeling of mononucleosomes by the yeast SWI/SNF remodeler, RSC. We observed that RSC primarily translocates DNA around the nucleosome without substantial displacement of the H2A-H2B dimer. At the sites where DNA enters and exits the nucleosome, the DNA moves largely along or near its canonical wrapping path. The translocation of DNA occurs in a stepwise manner, and at both sites where DNA enters and exits the nucleosome, the step size distributions exhibit a peak at approximately 1–2 bp. These results suggest that the movement of DNA across the nucleosome is likely coupled directly to DNA translocation by the ATPase at its binding site inside the nucleosome.