Pooling strategy for second cohort samples. Example: Normalized DNA samples from column 12 of plates 1 and 2 as well as samples from plate 3, column 12, rows A, B, C, and D are pooled together to form pool 12. Normalized DNA samples from column 1 of plates 4 and 5 as well as samples from plate 3, column 1, rows E, F, G, and H are pooled together to form pool 13.