The aim of this letter is to provide the scientific basis of the successful prognosis observed in Acanthamoeba keratitis patients in the absence of anti-Acanthamoeba agents. Patients were treated for photophobia with anticholinergics (Atropine), and for inflammation and pain, they were given topical Diclofenac sodium. The patients responded well with the aforementioned combination. It was suggested that the successful prognosis was due to suppression of the inflammation. Here, we explained that anticholinergic agent showed potent anti-amoebic effects. Thus the successful prognosis observed in AK cases were not merely due to regulation of the inflammatory process, but were most likely due to amoebicidal effects of anticholinergic agent.