In this work, we present the study of the behavior of 15 tomato cultivars under different grow lights to evaluate the quality of seedlings in the production system. The lamps used are: compact fluorescent, high-efficiency fluorescent, fluorescent, and pure blue light-emitting diodes (B-LEDs). The trial was carried out in a culture chamber with the temperature and relative humidity continuously controlled. Spectral radiation was measured at the canopy level. The following were quantified: fresh, dry biomass partitioning organs (leaves, stems, and roots), the total dry weight/total fresh weight relationship, shoot/root ratio, and indole acetic acid. We found high-efficiency fluorescent light treatment has a very interesting spectral quality for all cultivar applications as a result of it having the lowest photosynthetically active radiation (PAR):near infrared (NIR), blue:red (B:R), blue:far red (B:FR), and red:far red (R:FR) ratios; ‘Conquista’, ‘Velasco’, and ‘Lynna’ are cultivars that show sensitivity to special wavebands (ultraviolet, B, R, and FR). ‘Ikram’, ‘Saladar’, and ‘Delizia’ tolerate the lack of minimum energy and spectral quality.