Figure S1: Immunodetection of efflux pumps Pgp, MRP1 The multidrug-resistant doxorubicin-selected MCF7-R breast cancer cell line was used as a positive control for Pgp and MRP1 [7]. Proteins from the extracts (105 cells per lane) were electrophoretically separated on 7.5% SDS-PAGE. Primary antibody used were anti-p-Glycoprotein clone F4 (Neomarkers, Fremont, CA) and anti-MRP1 (Alexis Corp, San Diego, CA). Equal loading is shown by β-tubulin (clone tub2.1, Sigma).