The transport and deposition of terrestrially derived organic matter (TOM) into lake and ocean is a key but poorly constrained aspect of the modern global carbon cycle. An attempt has been done for estimating a transport time of TOM from the drainage basin of Lake Biwa, the largest lake in Japan. We have determined the 14C contents of the lignin-enriched fraction of the core sediment from the central part of Lake Biwa. The age of lignin-enriched fraction at the deposition time was estimated to be 7.5×103 years for the last glacial interval. Even in Lake Biwa with more than 100 rivers from the relatively small drainage basin (3850km2), TOM was transported at very long time (>103years).