Lichens are organisms that have emerged about four hundred million years ago and during the Devonian period. Lichens are complex organisms that are composed of a fungus and a photosynthetic partner that live in a symbiotic. Lichens have a wide distribution and are compatible with any type of weather conditions. These organisms grow well on calcium stones, and have destructive effects of physical and chemical on mineral compositions in rocks. Since lichens live on stone surfaces and old buildings, they can do irreparable damages to these structures. In recent years, multi-purpose methods has been developed as biocides for lichen removal of historic stone surfaces. These methods in addition to cleaning and conservation of biodeterioration of stoneworks, also can the lichens bio-protect as a part of nature and ecosystems.