Plasmid construction for protein expression. cDNA encoding for S100A8, S100A9, S100A12 or fusion chimera proteins were introduced in pUCP20, pGEX5x2 or pIVEX2.4d plasmids using the indicated restriction enzyme. Protein expression was carried out in Pseudomonas aerugina (1) or Escherichia coli (2). (DOC)