The potential use of non-tumorigenic agrobacteria for the biological control of grapevine crown gall in Italy was investigated. Four Agrobacterium strains belonging to the species radiobacter and vitis were used to protect the susceptible cv. Malvasia Istriana grafted on the rootstock 420 A. Moreover, the effect of each treatment on grapevine vitality and growth was assessed, including the percentage of marketable vines, as determined by industry standards. Treatments with the antagonists clearly reduced tissue colonization by the pathogen, with a drop of more than 100-fold in pathogen populations in the samples collected at the graft point. Another important effect was the reduction of internal necrosis possibly induced by the high concentration of the nopaline strain CG 49 used in the experiments. According to viticultural and commercial parameters, treatments with the antagonists improved the quality of the vines, with fewer discards and a high percentage of marketable material. Therefore, these antagonists can be considered beneficial for grapevine.