The binding ability and hemagglutination activity of the rPnxIIIA variants. (A) Coomassie blue-stained SDS-PAGE analysis of rPnxIIIA variants. Lanes: M, protein ladder; 1, wild-type rPnxIIIA; 2, rPnxIIIA209; 3, rPnxIIIA197; 4, rPnxIIIA151. (B) Ability of rPnxIIIA variants (10 μg/ml) to bind to the rat collagen type I measured by A620. Numbers are represented as follows: 1, wild-type rPnxIIIA; 2, rPnxIIIA209; 3, rPnxIIIA197; 4, rPnxIIIA151. (C) Changes in hemagglutination activity of different concentration of the rPnxIIIA variants with sheep erythrocytes. Numbers are represented as follows: 1, rPnxIIIA209; 2, rPnxIIIA197; and 3, rPnxIIIA151.