Survivin expression in HUVEC, melanocytes and SK-MEL-110. Human epidermal melanocytes NHEM-neo (Lonza) were grown in MBM-2 supplemented with MGM-4 SingleQuots (CaCl2, FGF-2, PMA, rh-Insulin, Hydrocortisone, BPE, FBS and Gentamicin/Amphotericin-B) (Lonza). Human umbilical vein endothelial cells (HUVECs; Lonza) were maintained in EBM-2 medium (Lonza) supplemented with endothelial growth medium 2 (EGM-2) kit (FCS, hydrocortisone, hFGF-B, VEGF, R3-IGF-1, ascorbic acid, hEGF, GA-1000, heparin), according to manufacturer's instructions. Cells were cultured at 37°C in a 5% CO2 atmosphere. Survivin expression in three different cell types (HUVEC, NHEM-Neo or SK-MEL-110) was examined by western blotting. β-actin was used as control of equal loading. One representative experiment was reported, while the quantification refers to 3 different experiments.