Rapid bioaerosol characterization has immediate applications in the military, environmental and public health sectors. Recent technological advances have facilitated single particle detection of fluorescent aerosol in near real-time; this leverages controlled exposures with single or multiple ultraviolet wavelengths, followed by the characterization of associated fluorescence. This type of Ultravio let induced fluorescence has been used to detect some intact airborne microorganisms in laboratory studies, and has been extended to field studies which implicate bioaerosol to compose a substantial fraction of supermicron atmospheric particles. To enhance the information yield which new generation fluorescence instruments provide, we report the compilation of a systematic referential catalogue including more than fifty pure cultures of common airborne bacteria, fungi and pollens. This catalogue juxtaposes intrinsic optical properties and multiple bandwidths of fluorescence spectra, which manifest to clearly distinguish between major classes of airborne microbes and pollen.