Sup. Fig. 1. Interactions of BD M4 mutant Gag3 D60A/R63A with Gag3 wild type, D60A/R63A, G87A, F93A, E148A/K149A, and E190A/R191A. The D60A/R63A mutation disrupts interactions with wild type and other mutant Gag3 proteins. Images of yeast strain yAH109 containing two-hybrid pGAD or pGBK vectors or expression plasmids as described in text. Cells are shown as representative streaks of independent transformants growing on synthetic dextrose medium lacking tryptophan, leucine, and adenine. Mutants are M4 (D60A/R63A), MHR2 (G87A), MHR4 (F93A), M13 (E148A/K149A), and M18 (E190A/R191A). Binding domain (BD) fusions are labeled in the top row and shown in columns; activation domain (AD) fusions are labeled in the left column and shown in rows. Scoring is based on four individual transformants (two shown). There was variability among individual BD-Gag3/AD-N-terminal domain (NTD) transformants (for example Figure 1, Additional file 7 and Additional file 10).