Representative flow cytometry images that quantify results presented in Figure 4d, main text. The 10 day old subcutaneous matrigel plugs (n = 5) containing BGO1 hESCs derived SSEA3+/ABCG2+ cells [obtained either before hypoxia (ABCG2+nox) or after day-4 post-hypoxia (ABCG2+hox)] were subjected to enzymatic dissociation 22 and staining with antibody against Nanog28 followed by flow cytometry (5,000 single-cell events were collected). Whereas the ABCG2+nox matrigel plugs contained 3.1 ± 0.5 percentage of Nanog positive cells, the ABCG2+hox matrigel plugs contained 23.5 ± 1.2 percentage of Nanog positive cells (n = 5, *p<0.0001).