Objectives : Genotyping of Salmonella strains is an important molecular tool to discriminate among isolates and to improve epidemiological studies when an outbreak occurs. Methods : Among the DNA-based methods of genotyping, pulsed field gel electrophoresis (PFGE) is currently in use to subtype Salmonella isolates. In this study we evaluated the feasibility of genotyping of Salmonella Infantis by XbaI restriction. Separation of restricted fragments was performed by PFGE. To test the possibility to apply this methodology to epidemiological investigation, a collection of 26 Salmonella Infantis strains isolated in different regions of Casablanca Morocco were analyzed. Results : The results of antibiotic susceptibility tests showed that 84,6% of Salmonella isolated were sensitive to all antibiotics tested and 19,24% isolates have a low level of resistance to beta-lactams. Only one strain has a class 1 integron with 0.8 kb size. Five different PFGE profiles were defined for XbaI digestion. Conclusion : In conclusion The PFGE method demonstrated an adequate typing ability and represent a powerful tool to discriminate within the Salmonella Infantis serovar.