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BioMed Central, Genetics Selection Evolution, 6(34), p. 649

DOI: 10.1186/1297-9686-34-6-649

BioMed Central, Genetics Selection Evolution, 6(34), p. 649-656

DOI: 10.1051/gse:2002029

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Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding

Journal article published in 2002 by Viviana Rodriguez, Silvia Llambí, Silvia Llamb�, Alicia Postiglioni, Karina Guevara, Gonzalo Rincón ORCID, Gonzalo Rinc�n, Gabriel Fernández, Gabriel Fern�ndez, Beatriz Mernies, María Victoria Arruga, Arruga Mv
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Fragile sites (FS) seem to play a role in genome instability and may be involved in karyotype evolution and chromosome aberrations. The majority of common fragile sites are induced by aphidicolin. Aphidicolin was used at two different concentrations (0.15 and 0.30 microM) to study the occurrence of FS in the cattle karyotype. In this paper, a map of aphidicolin induced break points and fragile sites in cattle chromosomes was constructed. The statistical analysis indicated that any band with three or more breaks was significantly damaged (P<0.05). According to this result, 30 of the 72 different break points observed were scored as fragile sites. The Pearson correlation test showed a positive association between chromosome length and the number of fragile sites (r=0.54). On the contrary, 21 FS were identified on negative R bands while 9 FS were located on positive R bands.