Microbial lipases have great industrial interest. However, few studies explore the potential of endophytic fungi, micro-organisms that apparently not cause any damage to your host1, as production source of these enzymes. Our research group isolated many endophytic fungi associated with some plant species, which may represent important sources of promising lipases. The monoglycerides (MAGs) are amphiphilic molecules that have applications such as emulsifiers, solubility agents and chiral building blocks. They are currently produced by alkaline glycerolysis oil under pressure and elevated temperature, resulting in low yields and numerous by-products. The use of enzymatic processes catalyzed by lipase may lead to a more ecological approach2. This work aimed to trace the lipase activity of endophytic fungi for use as biocatalysts in esterification reactions to produce MAGs.