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National Academy of Sciences, Proceedings of the National Academy of Sciences, 45(102), p. 16182-16187, 2005

DOI: 10.1073/pnas.0502828102

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Determination of cell survival by RING-mediated regulation of inhibitor of apoptosis (IAP) protein abundance.

Journal article published in 2005 by John Hendry Silke ORCID, Diep Chu, Tobias Kratina, Paul G. Ekert ORCID, Catherine L. Day, Miha Pakusch, David C. S. Huang, David L. Vaux ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Inhibitor of apoptosis (IAP) proteins, which bind to caspases via their baculoviral IAP repeat domains, also bear RING domains that enable them to promote ubiquitylation of themselves and other interacting proteins. Here we show that the RING domain of cIAP1 allows it to bind directly to the RING of X-linked IAP, causing its ubiquitylation and degradation by the proteasome, thus revealing a mechanism by which IAPs can regulate their abundance. Expression of a construct containing the RING of cellular IAP1 was able to deplete melanoma cells of endogenous X-linked IAP, promoted apoptosis, and also markedly reduced their clonogenicity when treated with cisplatin. Cross control of protein levels by RING domains may therefore enable their levels to be manipulated therapeutically.