5′-non-coding genome region (5′-ncr) of European and African rodent hepaciviruses. A. 5′-end of RMU10-3382 (GenBank, KC411777). This structure was mostly related to the Pegivirus type 4 IRES. Nucleotides conserved with other pegiviruses are marked in red, paired compensatory substitutions in NLR-365 (KC411796) and the partially available NLR-AP70 5-UTR (KC411784) that support the structure are in green. The Ia loop is very similar in length and shape to HCV and GBV-B. The start codon is boxed in red, additional non-functional start codons between the poly-pyrimidine stretch typical for pegiviruses and the true start codon are boxed in blue. The binding site for microRNA-122 is underlined. B. 5′-end of SAR-46 (KC411807). This structure was mostly related to a Hepacivirus type 3 IRES. Nucleotides conserved with HCV are marked in red. The slippery site is underlined. The start codon is boxed. Stem-loop structures in both foldings are numbered according to Pegi- and Hepacivirus reference strains. (TIF)