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American Chemical Society, Journal of the American Chemical Society, 44(132), p. 15522-15524, 2010

DOI: 10.1021/ja107677q

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Interaction-Dependent PCR: Identification of Ligand−Target Pairs from Libraries of Ligands and Libraries of Targets in a Single Solution-Phase Experiment

Journal article published in 2010 by Lynn M. McGregor ORCID, David J. Gorin, Christoph E. Dumelin, David R. Liu
This paper is available in a repository.
This paper is available in a repository.

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Abstract

Interaction-dependent PCR (IDPCR) is a solution-phase method to identify binding partners from combined libraries of small-molecule ligands and targets in a single experiment. Binding between DNA-linked targets and DNA-linked ligands induces formation of an extendable duplex. Extension links codes that identify the ligand and target into one selectively amplifiable DNA molecule. In a model selection, IDPCR resulted in the enrichment of DNA encoding all five known protein-ligand pairs out of 67 599 possible sequences.