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Nature Research, Nature Communications, 1(7), 2016

DOI: 10.1038/ncomms10534

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Volta phase plate cryo-EM of the small protein complex Prx3

Journal article published in 2016 by Maryam Khoshouei, Mazdak Radjainia, Amy J. Phillips, Juliet A. Gerrard, Alok K. Mitra ORCID, Jürgen M. Plitzko, Wolfgang Baumeister, Radostin Danev
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

AbstractCryo-EM of large, macromolecular assemblies has seen a significant increase in the numbers of high-resolution structures since the arrival of direct electron detectors. However, sub-nanometre resolution cryo-EM structures are rare compared with crystal structure depositions, particularly for relatively small particles (<400 kDa). Here we demonstrate the benefits of Volta phase plates for single-particle analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 dodecamers at 4.4 Å resolution. The Volta phase plate improves the applicability of cryo-EM for small molecules and accelerates structure determination.