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American Chemical Society, Organic Letters, 11(13), p. 2888-2891, 2011

DOI: 10.1021/ol200909j

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A Direct, Efficient Method for the Preparation of siRNAs Containing Ribo-likeNorthBicyclo[3.1.0]hexane Pseudosugars

Journal article published in 2011 by Montserrat Terrazas, Anna Aviñó, Maqbool A. Siddiqui, Victor E. Marquez, Ramon Eritja ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.