We report the first record of Nymphaea belophylla for the Pantanal wetland in the State of Mato Grosso do Sul, Brazil, being the southernmost record of this waterlily. It is one of the seven neotropical species of this genus in the Pantanal. We collected N. belophylla during a study on seasonality of herbaceous species composition in a remote area of the Paraguay river floodplain, what reinforces the importance of floristic surveys to improve knowledge on species distribution.