Temperature in Eagle Cave, central Spain, was measured over a year to determine the effect of tourists on the natural environment. The mean cave temperature was 15.6°C in 2009, with a seasonal amplitude of <0.4°C. Access of tourists to the cavern produces thermal anomalies of <0.15°C, which are recovered overnight in most cases. During days with high visitor numbers, cumulative thermal anomalies may persist from one day to the next, causing an increase of cave temperature for longer periods. However, this anthropogenic effect disappears soon after the number of tourists reduces, lasting less than a week in most cases. Cumulative thermal anomalies are <0.02°C during most of the year and <0.1°C in periods with large number of visitors. The anthropogenic effect on cave temperature is non-persistent and has a small magnitude in comparison with natural oscillations. Thus, long-term absolute temperatures obtained from Eagle Cave are not affected by visitors. The input of thermal energy caused by tourists is absorbed as latent heat by the cave (causing evaporation), which prevents the increase of baseline temperatures in the environment. A condensation process occurs over cave walls and speleothems. This is the result of cooling the atmosphere during the thermal equilibration with cave walls once visitors leave. Although condensation is found in Eagle Cave, the magnitude of the process is not enough to cause any significant condensation corrosion that could damage speleothems as a result of the tourist visits. The cave is in thermal equilibrium with surface temperatures, and calibration studies will produce suitable results for palaeoclimate studies despite being a tourist cavern.