Published in

Elsevier, Journal of Structural Biology, 1(136), p. 20-29

DOI: 10.1006/jsbi.2001.4422

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Correlation of Topographic Surface and Volume Data from Three-Dimensional Electron Microscopy

Journal article published in 2001 by Eva Dimmeler, Roberto Marabini ORCID, Peter Tittmann, Heinz Gross
This paper was not found in any repository, but could be made available legally by the author.
This paper was not found in any repository, but could be made available legally by the author.

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Abstract

Three-dimensional(3D) reconstructions from tilt series in an electron microscope show in general an anisotropic resolution due to an instrumentally limited tilt angle. As a consequence, the information in the z direction is blurred, thus making it difficult to detect the boundary of the reconstructed structures. In contrast, high-resolution topography data from microscopic surface techniques provide exactly complementary information. The combination of topographic surface and volume data leads to a better understanding of the 3D structure. The new correlation procedure presented determines both the height scaling of the topographic surface and the relative position of surface and volume data, thus allowing information to be combined. Experimental data for crystalline T4 bacteriophage polyheads were used to test the new method. Three-dimensional volume data were reconstructed from a negatively stained tilt series. Topographic data for both surfaces were obtained by surface relief reconstruction of electron micrographs of freeze-dried and unidirectionally metal-shadowed polyheads. The combined visualization of volume data with the scaled and aligned surface data shows that the correlation technique yields meaningful results. The reported correlation method may be applied to surface data obtained by any microscopic technique yielding topographic data.