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BioMed Central, Cancer Cell International, 1(14), 2014

DOI: 10.1186/1475-2867-14-1

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The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

Journal article published in 2014 by I.-Ling Lin, Han-Lin Chou, Jin-Ching Lee, Feng-Wei Chen, Yao Fong, Wei-Chiao Chang, Hurng Wern Huang, Chang-Yi Wu, Wen-Tsan Chang, Hui-Min David Wang, Chien-Chih Chiu ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

Abstract The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.