Sirtuin-3 (SirT3) is a member of the sirtuin family of nicotinamide adenine dinucleotide (NAD)-dependent deacetylases. SirT3 deacetylates mitochondrial acetylated proteins, then increases the activities of ROS scavenger enzyme and modulates mitochondrial respiratory function, which provide an inhibitory effect on accumulation of reactive oxygen species (ROS). This review summarizes and introduces the amelioratory effect of SirT3 on oxidative stress-induced functional and organic damage, and provides beneficial references to SirT3 in related pathological and physiological mechanisms for the future researches.