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American Society for Microbiology, Antimicrobial Agents and Chemotherapy, 1(60), p. 640-645, 2016

DOI: 10.1128/aac.01920-15

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Development of an intracellular screen for new compounds able to inhibit Mycobacterium tuberculosis growth in human macrophages

Journal article published in 2015 by Flavia Sorrentino, Ruben Gonzalez Del Rio, Xingji Zheng, Jesus Matilla Presa, Pedro Torres Gomez, Pedro Torres Gomez, Maria Martinez Hoyos, Maria Esther Perez Herran, Maria Esther Perez Herran, Alfonso Mendoza Losana, Yossef Av-Gay ORCID
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.