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A reliable ex vivo invasion assay of human reticulocytes by Plasmodium vivax
Journal article published in 2011 by
Bruce Russell,
Rossarin Suwanarusk,
Céline Borlon,
Ft T. M. Costa,
Cs S. Chu,
Mj J. Rijken 
,
Kanlaya Sriprawat,
Lucile Warter,
Eg G. L. Koh,
Benoit Malleret,
Yves Colin,
Olivier Bertrand,
Jh H. Adams ,
Umberto D'Alessandro,
Georges Snounou
and other authors.
,
Kanlaya Sriprawat,
Lucile Warter,
Eg G. L. Koh,
Benoit Malleret,
Yves Colin,
Olivier Bertrand,
Jh H. Adams ,
Umberto D'Alessandro,
Georges Snounou
and other authors.
Abstract
Currently, there are no reliable red blood cells invasion assays to guide the discovery of vaccines against Plasmodium vivax, the most prevalent malaria parasite in Asia and South America. Here we describe a protocol for an ex vivo P. vivax invasion assay that can be easily deployed in laboratories located in endemic countries. The assay is based on mixing enriched cord blood reticulocytes with matured, trypsin treated P. vivax schizonts concentrated from clinical isolates. The reliability of this assay was demonstrated using a large panel of P. vivax isolates freshly collected from patients in Thailand.