Hexaoligochitin produced by chitinase, ASCHI61, from Aeromonas schubertii was recently expressed. In this work, the optimal conditions for the mass production of ASCHI61 were investigated. The efficiency of recombinant protein expression in Escherichia coli was determined by various parameters, including the pH of the culture medium, induction temperature, shaking speed, inducer concentration, and induction period. The optimization experiments could be simplified through a statistical design of experiments (response surface methodology). From the fractional factorial design, the interactive effect of induction temperature and time was the most significant. The total activity of the enzyme was 32,092 U at 23.9°C with 115min of induction. Under those conditions, the total activity of the recombinant protein was 30,650 U in the fermentation experiments, with an error of only 4.8%. The total activity of ASCHI61 increased 1.54-fold under the optimal conditions. Based on the results, ASCHI61 can be expressed more for hexaoligochitin production.