A novel halotolerant actinomycete, designated strain BNT52^T, was isolated from soil collected from Cihanbeyli Salt Mine in the central Anatolia region of Turkey, and examined using a polyphasic taxonomic approach. The isolate was found to have chemical and morphological properties typical of the genus Amycolatopsis and formed a distinct phyletic line in the 16S rRNA gene tree. Strain BNT52^T was most closely related to Amycolatopsis nigrescens CSC17Ta-90^T (96.7 %), Amycolatopsis magusensis KT2025^T (96.6 %), Amycolatopsis sulphurea DSM 46092^T (96.6 %), Amycolatopsis dongchuanensis YIM 75904^T (96.5 %), Amycolatopsis ultiminotia RP-AC36^T (96.4 %) and Amycolatopsis sacchari DSM 44468^T (96.4 %). Sequence similarities with other strains of species of the genus Amycolatopsis were lower than 96.2 %. The isolate grew at 20–37 °C, pH 6–12 and in the presence of 0–10 % (w/v) NaCl. The cell wall of the novel strain contained meso-diaminopimelic acid and arabinose and galactose as the diagnostic sugars. Major fatty acids were iso-C_{16 : 0} 2-OH and iso-C_{16 : 0}. The predominant menaquinone was MK-9(H₄). The polar lipids detected were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylmethylethanolamine. The genomic DNA G+C content was 68.8 mol%. On the basis of the data from this polyphasic taxonomic study, strain BNT52^T represents a novel species within the genus Amycolatopsis for which the name Amycolatopsis cihanbeyliensis sp. nov. is proposed (type strain BNT52^T = KCTC 29065^T = NRRL B-24886^T = DSM 45679^T).