We have developed a scanning magnetic microscope (SMM) with 25 µm resolution in spatial position for the magnetic features of room temperature objects. The microscope consists of a high-temperature superconductor (HTS) dc SQUID sensor, suspended in vacuum with a self-adjusting standoff, close spaced liquid nitrogen Dewar, X–Y scanning stage and a computer control system. The HTS SQUIDs were optimized for better spatial and field resolutions for operation at liquid nitrogen temperature. Measured inside a magnetic shield, the 10 pT Hz−1/2 typical noise of the SQUIDs is white down to frequencies of about 10 Hz, increasing up to about 20 pT Hz−1/2 at 1 Hz. The microscope is mounted on actively damped platforms, which negate vibrations from the environment as well as damping internal stepper motor noises. A high-resolution video telescope and a 1 µm precision z-axis positioning system allow a close positioning of the sample under the sensor. The ability of the sensors to operate in unshielded environmental conditions with magnetic fields up to about 15 G allowed us to perform 2D mapping of the local ac and dc susceptibility of the objects.