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American Institute of Physics, Biomicrofluidics, 4(8), p. 046505

DOI: 10.1063/1.4894409

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A polystyrene-based microfluidic device with three-dimensional interconnected microporous walls for perfusion cell culture

Journal article published in 2014 by Chung Yu Chan ORCID, Vasiliy N. Goral, Michael E. DeRosa, Tony Jun Huang ORCID, Po Ki Yuen ORCID
This paper is available in a repository.
This paper is available in a repository.

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Abstract

In this article, we present a simple, rapid prototyped polystyrene-based microfluidic device with three-dimensional (3D) interconnected microporous walls for long term perfusion cell culture. Patterned 3D interconnected microporous structures were created by a chemical treatment together with a protective mask and the native hydrophobic nature of the microporous structures were selectively made hydrophilic using oxygen plasma treatment together with a protective mask. Using this polystyrene-based cell culture microfluidic device, we successfully demonstrated the support of four days perfusion cell culture of hepatocytes (C3A cells).