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Nature Research, Scientific Reports, 1(5), 2015

DOI: 10.1038/srep09570

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A cost-effective RNA sequencing protocol for large-scale gene expression studies

Journal article published in 2015 by Zhonggang Hou, Peng Jiang ORCID, Scott A. Swanson, Angela L. Elwell, Jennifer M. Bolin, Bao Kim S. Nguyen, Ron Stewart, James A. Thomson
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

AbstractRNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.