Specificity controls for antibodies used in ChIP. Representative gel analysis of ChIP results indicating the specificity of the antibodies for the histone modification analysis in this study. Antibodies specific to H3K9me2, H3K9me3, H3K27me2, and H3K27me3 show enrichment for H3K9me3 and H3K27me3 at the DMD. Positive control PCRs for H3K9me2 and H3K27me2 (Charlie [1]), H3K9me3 (Actin), and H3K27me3 (Hoxa9 [2]) are included as well as a test for the Rasgrf1 DMD. NTC, no template control; WCE, whole cell extract not immunoprecipitated; no ab, mock precipitations done without antibody. (0.12 MB TIF)