Objective: The objective of this study was to isolate and characterize the bioactive principles from the aqueous ethanolic extract of flower buds of Eugenia caryophyllus. Methods: The isolation was done using column chromatography using gradient elution with different mobile phases. The isolated compound was subjected to spectral analysis. Structure elucidation was carried out on basis of spectral analysis. Results: The infra-red spectra showed specific absorption bands for flavanoids viz. 1245.86 to 1111.01 cm -1 for HCO stretch of ether; 1330 to 1050 cm -1 for CO stretch of lactone. In addition IR spectrum showed specific absorption bands for flavanoids viz. 3432.57cm -1 for OH stretch for alcohol and phenol. Mass spectra showed pseudomolecular ion (M + ion) peak at m/z 464 which corresponds to molecular formula C21H20O12 Conclusion: From the spectral characteristics, the isolated compound from the extract was confirmed to be Gossypetin 7-O rhamnopyranoside (Rhodiolgin). .