The effects of boron (B) fertilizer applied 10 growing seasons earlier were studied in mature Norway spruce (Picea abies (L.) Karst.) trees in long-term factorial fertilization experiments at two field sites. Needle nutrient status, above-ground and below-ground growth and and carbon concentrations in the annual rings were measured. Needle B concentrations varied between 4 and 19 mg kg−1 on the plots that had not received B fertilization. On the B-fertilized plots they varied between 15 and 39 mg kg−1. The lowest B concentrations were on the plots that had received N or NCa fertilization. Needle Mn and Zn concentrations were lower on the B plots than on the plots that had not received B fertilization, although not significantly. Mean annual volume growth was slightly higher on the B plots at the more fertile site, but not at the less fertile one. The living:dead fine root mass ratio and living fine root length were also higher on the B-fertilized plots than on the unfertilized plots, but was not significantly affected, suggesting that the water status of the trees was not markedly altered by the increase in root growth. The carbon concentration in the annual rings was higher in the B-fertilized trees than in the unfertilized ones, suggesting the importance of B for wood formation.