American Society for Microbiology, Applied and Environmental Microbiology, 12(76), p. 4096-4098, 2010
DOI: 10.1128/aem.02797-09
Full text: Download
ABSTRACT Short-chain alcohol dehydrogenase, encoded by the gene Tsib_0319 from the hyperthermophilic archaeon Thermococcus sibiricus , was expressed in Escherichia coli , purified and characterized as an NADPH-dependent enantioselective oxidoreductase with broad substrate specificity. The enzyme exhibits extremely high thermophilicity, thermostability, and tolerance to organic solvents and salts.