Korean Chemical Society, Bulletin- Korean Chemical Society, 11(31), p. 3103-3108
DOI: 10.5012/bkcs.2010.31.11.3103
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Conducting polymers (CPs) are widely used as matrixes for the entrapment of enzymes in analytical chemistry and biosensing devices. However, enzyme-catalyzed polymerization of CPs is rarely used for immunosensing due to the difficulties involved in the quantitative analysis of colloidal CPs in solution phase. In this study, an enzyme-amplified electrocatalytic immunosensor employing a CP as a redox marker has been developed. A polyanionic polymer matrix, α-amino-ω-thiol terminated poly(acrylic acid), was employed for precipitation of CP. The acrylic acid group acts as a polyanionic template. The thiol terminus of the polymer was used to produce self-assembled monolayers (SAMs) on Au electrodes and the amine terminus was employed for immobilization of biomolecules. In an enzyme-amplified sandwich type immunosensor, the polyaniline (PANI) produced enzymatically is attracted by the electro-static force of the matrix polymer. The precipitated PANI was characterized by electrochemical methods.