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American Society for Microbiology, Journal of Clinical Microbiology, 2(54), p. 474-477, 2016

DOI: 10.1128/jcm.02635-15

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Does the Capsule Interfere with Performance of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Identification of Cryptococcus neoformans and Cryptococcus gattii?

Journal article published in 2016 by Thomaz Dy, João N. de Almeida Júnior ORCID, Danilo Y. Thomaz ORCID, Rafaella C. Grenfell, Monica S. M. Vidal, Grenfell Rc, Vidal Ms, Mauro C. Giudice, Giudice Mc, Gilda M. B. Del Negro ORCID, Del Negro Gm, Luiz Juliano, Gil Benard, J. N. De Almeida, de Almeida Júnior Jn
This paper is made freely available by the publisher.
This paper is made freely available by the publisher.

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Abstract

ABSTRACT We described the impact of the capsule size for Cryptococcus neoformans and Cryptococcus gattii identification at the species level by Bruker matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS). After experimental capsule size modulation, we observed that reducing the capsule size resulted in improved identification by Bruker MALDI-TOF MS across all of the reference strains analyzed.