This study was carried out to determine the effects of different levels of Yucca schidigera spraying in different litter materials on some litter traits (moisture, pH, ammonia, total colony count, number of Enterobacteriaceae and number of yeast and mold) and breast burn of broilers at the 5th week of production. A total of four hundred thirty two 1-d-old male broiler chicks (ROSS-308) were used. In this study 12 chicks were put in each pen having 170x94x90 cm (depth x length x height). Half of the litter was wood shavings, the other was rice hull. Yucca schidigera extract was pulverized weekly at the level of 0, 4% and 8% to each pen from the second week of the study. Litter materials and Yucca schidigera spraying at different level did not affect the examined litter traits and breast burns of broilers (P>0.05) at the 5th week of production.