Introduction Leishmaniasis constitute a group of parasitic diseases caused by protozoan parasites of the genus Leishmania. We have developed a species-specific L. infantum LAMP assay for the diagnosis of clinical canine leishmaniasis using the cysteine protease B (cpb) multi-copy gene as target. This technique was compared to serological, PCR and parasitological diagnostics. Materials and Methods -The LAMP primer sets were designed using Primer Explorer Ver.4. -The L. infantum specific LAMP reaction was standardized for optimal temperature and time. -Seventy-five DNAs extracted from Blood of suspicious Leishmania infected dogs from different zones in and around Tunis. -The dogs were examined for occurrences of visible clinical signs of the disease. -The samples were tested by : Microscopy (Gold standard), Serology (IFAT), PCR and LAMP. -Statistical Analysis. Results 1. Sensitivity and Specificity of the L. infantum specific LAMP A set of oligonucleotide primers targeting L. infantum cpb genes sequences were designed for LAMP reaction. Serially diluted samples were assessed for the presence of the L. infantum strain (MHOM/TN/80/IPT1) promastigote DNA used as reference. LAMP was able to detect up to 50 fg of DNA against up to 100 pg detected by conventional cpb PCR (Chaouch et al., 2013). To evaluate the specificity of the LAMP reaction, DNA samples from other Leishmania and Trypanosoma species (L. major, L. tropica, L. turanica, L. gerbilii, L. tarentolea and T.cruzi) were examined. No amplification product was detected even when using up to 50 ng of DNA showing that the LAMP cpb was specific for L. infantum detection (data not shown). Acknowledgments : This study received financial support from the International Atomic Energy Agency (IAEA Contract n°15137 -Contract n°15111) and from Ministère de l'Enseignement et de la Recherche Scientifique, Tunisia LR00SP04).